By H.S Majewski, J.F Klaverkamp, D.P Scott,
Water Research, Volume 12, Issue 4, 1978, Pages 217-221, ISSN 0043-1354, DOI: 10.1016/0043-1354(78)90089-1.

Acute lethality and sub-lethal effects of acetone, ethanol, and propylene glycol on the cardiovascular and respiratory systems of rainbow trout (Salmo gairdneri) were examined. The 24 h LC50 values for acetone and ethanol in a flow-through bioassay system at 10[degree sign]C +/- 0.5, are 6100 mg l-2 and 11,200 mg l-1, respectively. No mortality to fingerling trout was produced by propylene glycol at 50,000 mg l -1 during a 24 h exposure period in a static system.
Acetone and ethanol, at about 0.48 and 0.26 of the fingerling LC50, respectively, affected cardiovascular/respiratory parameters in adult rainbow trout. Acetone produced an increase in ventilation rate to a maximum of 158% of control values, as well as an increase in buccal pressure amplitude attaining a maximum of 410% of control values. Ethanol exposed fish exhibited a slight depression in ventilation rate and buccal pressure amplitude during initial stages of the 24 h exposure period. Ethanol had no effect on heart rate, despite a significant decrease in Q-T interval. Propylene glycol, at less than 0.08 of a concentration not producing apparent stress in fingerlings, had a mildly stimulatory effect on ventilation rate, and heart rate in adults. It is concluded that of the three solvents employed in this study, propylene glycol is most suitable for use as a solvent in fish toxicity tests.

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