Effects of tributyltin on salmon interrenal CYP11?, steroidogenic factor-1 and glucocorticoid receptor transcripts in the presence and absence of second messenger activator, forskolin

Publication year: 2010
Source: Marine Environmental Research, In Press, Corrected Proof, Available online 1 February 2010</br>
Trond M., Kortner , Nela, Pavlikova , Augustine, Arukwe</br>
The mechanisms by which TBT produces modulations of the endocrine systems are not fully described. In this study, juvenile salmon were force-fed diet containing TBT (0: solvent control, 0.1, 1 and 10mg/kg fish) for 72h. Subsequently, fish exposed to solvent control and 10mg/kg TBT were exposed to waterborne concentration of the adenyl cyclase stimulator forskolin (200?g/L) for 2 and 4h. Tissue and blood were sampled from individual fish (n=6). Gene expression patterns of CYP11?, steroidogenic factor-1 (SF-1), and glucocorticoid receptor (GlucR) were determined by qPCR. TBT generally decreased mRNA levels of CYP11?, GlucR and SF-1, compared to the solvent control...</br>

11th August 2010 12:14

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