Estrogenic effect of dioxin-like aryl hydrocarbon receptor (AhR) agonist (PCB congene
By Anne S. Mortensen, Augustine Arukwe
Marine Environmental Research, Volume 66, Issue 1, Pollutant Responses in Marine Organisms (PRIMO 14, Fourteenth International Symposium on Pollutant Responses in Marine Organisms (PRIMO 14), July 2008, Pages 119-120, ISSN 0141-1136, DOI: 10.1016/j.marenvres.2008.02.041.
Previous studies have shown that aryl hydrocarbon receptor (AhR) agonists possess anti-estrogenic activities and several mechanisms have been proposed to explain the interactions between AhR and estrogen receptor (ER) signalling pathways. In the present study, we show that 3,3'4,4'5-pentachlorobiphenyl (PCB126 - a dioxin-like AhR agonist) produced estrogenic responses in the absence of ER agonist, in fish in vitro system. We exposed salmon primary hepatocytes to PCB126 (1, 10 and 50 pM) and the ER agonist nonylphenol (NP; 5 and 10 [mu]M) singly and also in combination. Vitellogenin (Vtg) and zona radiata proteins (Zr-proteins) levels were analysed by semi-quantitative ELISA. We observed that the protein levels of Vtg and Zr-proteins were significantly induced in a concentration-specific manner in cells treated with PCB126 and NP, singly or in combination. In general, these results show a novel aspect of dioxin-like PCB effect not previously demonstrated in fish system.
Keywords: Dioxin-like PCB; Estrogenicity; Vitellogenin; Zona radiata protein