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11-08-2010, 02:52 PM #1
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Immunohistochemical localization of cytochrome P-450E in liver, gill and heart of scu
By Michael R. Miller, David E. Hinton, James J. Blair, John J. Stegeman
Marine Environmental Research, Volume 24, Issues 1-4, The Fourth International Symposium on Responses of Marine Organisms to Pollutants, 1988, Pages 37-39, ISSN 0141-1136, DOI: 10.1016/0141-1136(88)90250-4.
Monoclonal antibody directed against a major [beta]-naphthoflavone (BNF)-induced form of teleost cytochrome P-450, P-450E (equivalent to P-450c in rat) was used to immunolocalize this enzyme in liver, gill and heart of scup and trout. Liver sections from both species showed P-450E in the cytoplasm of hepatocytes. No regional differences were observed which might indicate zonation of cytochrome P-450E within subpopulations of hepatocytes. Scup exocrine pancreatic cells were only weakly positive. In the gill of both fish, cytochrome P-450E was restricted to the endothelium (pillar cells) of secondary lamellae, where fluorescence appeared as a chain in longitudinal sections through lamellae and as star-shaped clusters in en face views. Sections of ventricular wall in both species revealed P-450E was restricted to endothelium at margins of muscle bands limiting heart ventricular lumen. Localization in the specific cells of these and other organs may be fundamentally important in understanding the role of cytochrome P-450E.