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11-08-2010, 03:12 PM #1
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Metabolic conversion of cyclohexane by Pacific salmon microsomal preparations
By John M. Kennish, Colleen Montoya, Jane Whitsett, John S. French
Marine Environmental Research, Volume 17, Issues 2-4, Responses of marine organisms to pollutants, 1985, Pages 129-132, ISSN 0141-1136, DOI: 10.1016/0141-1136(85)90057-1.
Hydrocarbons are being introduced into the marine environment from a variety of sources including combustion processes, crude oil spills, fuel oil spills, and controlled disposal such as processed ballast water. Even in the relatively clean waters of Alaska hydrocarbons are being released at an alarming rate. The purpose of this study was to determine the optimum conditions for the metabolism of a representative hydrocarbon by Coho Salmon Oncorhynchus kisutch liver microsomes. The product of cyclohexane metabolism in the salmon microsomal system was identified by gas chromatography-mass spectrometry as cyclohexanol. Conditions of the microsome incubation were varied systematically to determine the optimum temperature, pH, and ionic strength for cyclohexanol production. Cyclohexanol was quantified by capillary column gas chromatography. Maximum cyclohexanol formation was achieved at 20[degree sign]C, a pH of 8[middle dot]0-8[middle dot]5 and an ionic strength of 0[middle dot]026. A linear rate of cyclohexanol formation is seen from 0-60 min of incubation and there is an apparent decrease in the rate from 60-90 min. Poor stability of the microsomal preparation from the species studied was also identified and several stability studies have been undertaken using cyclohexane metabolism as a monitor.