By A. Arukwe, T. Celius, B. T. Walther, A. Goksoyr
Marine Environmental Research, Volume 46, Issues 1-5, Pollutant Responses in Marine Organisms, July-December 1998, Pages 133-136, ISSN 0141-1136, DOI: 10.1016/S0141-1136(98)00002-6.

Induction of vitellogenin (Vtg) in males and juveniles of oviparous vertebrates has been used as a biomarker for xenoestrogens. Recently, we have demonstrated that synthesis of eggshell zona radiata proteins (Zrp) or zonagenesis is an integral aspect of fish oogenesis (Oppen-Berntsen et al. (1994) Journal of Experimental Zoology 268, 59-70), and that Zrp synthesis is a sensitive biomarker for nonylphenol (Arukwe et al. (1997) Environmental Health Perspective 105, 418-422). This study compares the responses of Vtg and Zrp in plasma of juvenile Atlantic salmon (Salmo salar) treated with 4-nonylphenol (NP) and o,p'-DDT (both at 25 mg kg-1, singly and in combination). Validated ELISA and immunoblot analysis show that Vtg and Zrp respond significantly stronger to NP treatment alone and in combination with o,p'-DDT compared to control and o,p'-DDT treatment alone. However, a slight reduction in NP-induced Zrp levels was indicated when NP was injected in combination with o,p'-DDT.

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