By Joseph B. Hunn, Steven J. Hamilton, Denny R. Buckler
Water Research, Volume 21, Issue 2, February 1987, Pages 233-238, ISSN 0043-1354, DOI: 10.1016/0043-1354(87)90055-8.

In a study designed to examine the long-term effects of inorganic selenium (IV) on early life stages of rainbow trout (Salmo gairdneri), survival was significantly reduced at selenium concentrations of 47 and 100 [mu]g l-1 after 90 days of exposure. Length and weight were significantly reduced after 90 days of exposure to 100 [mu]g l-1. Whole-body residues of selenium increased with increasing exposure concentrations but appeared to decline between 30 and 90 days of exposure. Analyses of trout backbone indicated little change in bone development with exposure to selenium (IV) with one exception; calcium concentrations were significantly decreased in fish exposed to [greater-or-equal, slanted] 12 [mu]g l-1 of selenium. Results of our study indicates that a recommended safe level of 10 [mu]g l-1 for inorganic selenium would not significantly affect growth and survival of rainbow trout; however, concentrations of selenium near this level can reduce the levels of calcium in the backbones of trout.
Keywords: inorganic selenium; Salmo gairdneri; mortality; growth; whole-body residues; bone composition

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